By combining immunocapture and fluorescence immunoassay, this E.coli O157:H7 test kit is able to provide both high sensitivity and specificity. Limit of detection using SignalyteTM-II is 10 cfu/ml.
Escherichia coli O157 Test Kit
Rapid and Sensitive Isolation and Identification of
E. coli O157:H7
- Isolation and concentration of E. coli O157:H7 from sample using immunoaffinity magnetic beads
- Detection using fluorometer, such as
- Creatv’s SignalyteTM-II spectrofluorometer
- 96 well fluorescent plate readers
Fluorescence immunoassay for the detection of a 10-fold serial E. coli O157:H7 dilution. (A) SignalyteTM-II; (B) 96 well fluorescence plate reader. The y-axis indicates the log10 fluorescence intensity (FI), while x-axis indicates log10 concentration of E. coli O157:H7 (cfu/ml). The threshold value of fluorescence intensity for the limit of detection is the average Fl plus 3 standard deviations of the negative control FI.