CellSieveTM CTC Enumeration Kit

Contains filters, buffers, and stains for 10 assays

For Research Use Only. This product is not intended for in vivo or diagnostic use. Performance, safety, and effectiveness have not been established and this kit is not approved by the FDA.

Standard Markers

  • DAPI (positive) for the nuclear
  • CK 8, 18, 19 (positive) to identify as epithelial cell
  • CD 45 (negative) to show it is not a blood cell

Fourth Fluorescent Channel

  • Monochrome
  • Standard kit: EpCAM
  • Can be customized

Camera requirement

  • Sensitive to NIR
  • 1.4M pixels minimum

Microscope Filter Set Specifications

Fluorescence Channel
Chroma Part #
Excitation Filter
Emission Filter
Dichroic Filter
DAPI
49000
350/50
460/50
400
FITC
49020
480/20
510/20
495
TRITC/PE
CellSieve-PE
546/10
585/40
565
Texas Red
49911
594/10
645/75
594
Cyanine5
CellSieve-Cy5
640/30
700/75
660

Materials

  • 10 CellSieveTM Microfilters
  • CellSieveTM Prefixation Buffer
  • CellSieveTM Postfixation Buffer
  • CellSieveTM Permeabilization Buffer
  • Ten White Gaskets and Ten Black Gaskets
  • CellSieveTM Enumeration Staining Solution
  • CellSieveTMMounting Solution with DAPI dilactate