Specifications for SignalyteTM-II


A New Age of Fluorescence Detection

SignalyteTM-II uses proprietary integrating waveguide technology to measure fluorescence signal in a low-cost disposable cuvette. Eight samples, plus one control, are tested in approximately one minute.


Description Ultra-sensitive spectrofluorometer with application software
Detection Modes Fluorescence spectrum
Fluorescence intensity
Luminescence
Read Type Endpoint
Kinetic
Sample format 9 x 35 µL disposable capillary cuvette
Light Source Wavelength-matched LED
Up to 4 LEDs per instrument
Wavelength UV (EX: 365 nm; EM: 400 nm long-pass)
Blue (EX: 470 nm; EM: 515 nm long-pass)
Amber (EX: 590 nm; EM: 630 nm long-pass)
Green (EX: 530 nm; EM: 570nm long-pass)
Red (EX: 635 nm; EM: 665 nm long-pass)
Note: EX is the peak wavelength of the LED, spectral width at ½ of the peak intensity varies with LED.
Multiplex Detection Up to 4 analytes
Emission Filters Long-pass color glass filters
Filter wheel allows up to 6 filters
Detector Cooled CCD spectrometer
Spectral Range 350 – 800 nmn
Limit of Detection* 0.9 atto-mol/cuvette fluorescein
0.09 atto-mol/cuvette Cy5
10 particles/cuvette SPHEROTM Purple nanoparticles (Spherotech, Inc.)
1 pg/mL dsDNA with PicoGreen
Dynamic Range* 6 logs (assay dependent)
Exposure Time 10 msec to 65 sec
Read Out Relative fluorescence
Dimensions (H x W x H) 249 mm (9.8") x 384mm (15.1") x 335mm (13.2")
Weight 13 kg (30 lbs)
User Interface Software to control instrument
Instrument connects to PC (not included) through USB port
Data Output Full spectrum
Bar-chart
Fluorescence intensity at user defined wavelength
Excel file export
Minimum System Requirements Windows® 7, Vista, or XP, Pentium 4 processor, 1 GB memory, CD-ROM drive, SVGA display, USB 2.0 port
Power 110-120 V/220-240 V, 50/60 Hz auto select, 3A
Included accessories One sample holder
Calibration tool samples
Application software CD
User manual
*Assay Dependent